Pathological Seminal Plasma Proteins by Cellulose Acetate Electrophoresis
نویسندگان
چکیده
منابع مشابه
Rapid cellulose acetate electrophoresis. I. Serum proteins.
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
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We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...
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In previous papers (Gavrilescu, Jellinek, and McMenemey, 1961, Gavrilescu and McMenemey, 1964) on comparative studies on the protein fractions in the serum and cerebrospinal fluid in multiple sclerosis we have noted increases in the °C2 and f globulins in the serum. Although not specific for this disease these changes are of interest, particularly as multiple sclerosis is considered to be purel...
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Patterns of creatine kinase (CK, EC 2.7.3.2) isoenzymes were studied in apparently healthy one- to 10-day-old neonates, by use of a sensitive fluorescent staining method with Sclavo CK-F/6001 reagent. Mean activities of CK3 (MM, 105 U/L), CK2 (MB, 6.8 U/L), CK1 (BB, 11 U/L), adenylate kinase (EC 2.7.4.3) anodal to CK3, and a fluorescent albumin artifact were found. Pooled plasma from neonates i...
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An immunoelectrophoretic procedure utilizing microprecipitation and cellulose acetate electrophoresis was developed for detection of antibody to specific virus. A model system of tobacco mosaic virus and homologous rabbit antiserum is described.
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 1981
ISSN: 0148-5016
DOI: 10.3109/01485018108987346